NEIL3 (Nei Endonuclease VIII Like Protein 3, FPG2, hFPG2, hNEI3, Endonuclease 8-like 3,DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Nei-like protein 3)

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£522.00

SKU:: 364329
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Val2-Val187 of mouse Neil3, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. The deduced 605aa protein has a calculated molecular mass of 67.9kD. Unlike NEIL1, NEIL3 contains a RAN-binding protein-like zinc finger motif and C-terminal zinc ribbon domains. Northern blot analysis of several human tissues detected a 2.4-kb transcript only in testis and thymus. Fluorescence-tagged NEIL3 colocalized with RPA2 in the nuclei of transfected HeLa cells and was excluded from nucleoli. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice