364302
NB1 (Neutrophil Specific Antigen 1, CD177, HNA2A, PRV1, HNA-2A, NB1 GP, Polycythemia Rubra Vera 1, Human neutrophil alloantigen 2a, Polycythemia rubra vera protein 1)
Size
£479.00
- SKU:
- 364302
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Cys133-Ser301of human NB1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- NB1, a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia. Independently, Kissel et al. (2001) cloned and characterized NB1, which they also referred to as CD177 and HNA2A. They obtained the cDNA after purification and microsequence analysis of NB1 protein from normal resting granulocytes. Kissel et al. (2001) noted amino acid differences between the PRV1 (Temerinac et al., 2000) and NB1 sequences at positions 3, 119, 323, and 379 and suggested that 2 different, highly homologous genes may exist. By genomic sequence analysis and PCR, Kissel et al. (2002) determined that the NB1 gene contains 9 exons. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice