364234
MTPN (Myotrophin, GCDP, V-1, Granule Cell Differentiation Protein)
Size
£537.00
- SKU:
- 364234
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Cys2-Gln118 of mouse MTPN, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Myotrophin, a 12kD protein, was originally isolated by Sen et al. (1990) from the hypertrophied ventricles of spontaneously hypertensive rats and later by Sil et al. (1993) from dilated cardiomyopathic human hearts. Mukherjee et al. (1993) found that myotrophin increases the transcript levels of protooncogenes as well as the transcript levels of beta-myosin heavy chain and ANF, markers for cardiac hypertrophy. The 117aa protein contained an acetylated N terminus and 2.5 internal 33aa ankyrin repeats. Sivasubramanian et al. (1996) identified a second half-ankyrin repeat in rat Mtpn. Northern blot analysis detected up to 7 transcripts between 0.4 and 4.3 kb expressed in all rat tissues examined. Highest levels were detected in brain, and lowest levels in skeletal muscle. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA:1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice