364230
MTF1 (Metal Regulatory Transcription Factor 1, MRE-binding transcription factor)
Size
£537.00
- SKU:
- 364230
- Purity:
- Purified by Protein A affinity chromatography and immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Gly2-Tyr139 of mouse MTF1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, containing 50% glycerol and 0.02% sodium azide.
- Extra Details:
- Metal regulatory transcription factor 1 is a transcription factor that induces expression of metallothioneins and other genes involved in metal homeostasis in response to heavy metals such as cadmium, zinc, copper, and silver. The protein is a nucleocytoplasmic shuttling protein that accumulates in the nucleus upon heavy metal exposure and binds to promoters containing a metal-responsive element (MRE). The human protein has 753 aa with 93% aa sequence identity to the mouse protein and has an extension of 78 aa at the C terminus without counterpart in the mouse. In the 2 species the factor has the same overall structure, including 6 zinc fingers in the DNA binding domain. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Immunohistochemistry: 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Positive Control Included: 364230A: MTF1, Positive Control Suppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol). Usage: 10ul per when DAB is the substrate; 5ul per well when used in ECL. Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice