364195
MOTS-c (Mitochondrial Open Reading Frame Of The 12S rRNA-c)
Size
£544.00
- SKU:
- 364195
- Purity:
- Purified by Protein A and peptide affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to MRWQEMGYIFYPRKLR from human MOTS-c, conjugated to OVA
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol
- Extra Details:
- Mitochondria are known to be functional organelles, but their role as a signaling unit is increasingly being appreciated. The identification of a short open reading frame (sORF) in the mitochondrial DNA (mtDNA) that encodes a signaling peptide, humanin, suggests the possible existence of additional sORFs in the mtDNA. A sORF within the mitochondrial 12S rRNA encoding a 16aa peptide named MOTS-c that regulates insulin sensitivity and metabolic homeostasis. Its primary target organ appears to be the skeletal muscle, and its cellular actions inhibit the folate cycle and its tethered de novo purine biosynthesis, leading to AMPK activation. MOTS-c treatment in mice prevented age-dependent and high-fat-diet-induced insulin resistance, as well as diet-induced obesity. These results suggest that mitochondria may actively regulate metabolic homeostasis at the cellular and organismal level via peptides encoded within their genome. Applications: Suitable for use in Immunocytochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice