364188
MMRN2 (Multimerin2, EMILIN3, EndoGlyx-1, Elastin Microfibril Interfacer 3, Elastin microfibril interface located protein 3, EndoGlyx-1 p125/p140 subunit)
Size
£537.00
- SKU:
- 364188
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Gln23-Ala188 of mouse MMRN2, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- ENDOGLYX1, a 500kD cell surface glycoprotein complex, and sequenced peptides derived from the component proteins. By searching databases using the peptide sequences, they obtained a full-length cDNA encoding MMRN2, which they called ENDOGLYX1. The deduced 949aa protein has a calculated molecular mass of 104.4kD. It contains an N-terminal signal sequence, followed by 2 major globular domains, an emilin (EM1) domain and a C-terminal C1q -like domain, which are separated by a large central coiled-coil-rich region. The EM1 domain contains 7 cysteines, 6 of which likely form disulfide bridges. The coiled-coil-rich region is divided into coiled-coil subdomains interrupted by noncoiled regions, allowing structural flexibility. An acidic region precedes the C1q region and is typically found in proteins that interact with glycosaminoglycans. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.5-2ug/ml Western Blot: 0.5-2ug/ml Immunohistochemistry: 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Positive Control Included: 364188A: MMRN2, Positive Control Suppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol). DAB: 10ul/well ECL: 5ul/well Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
- Shipping Conditions:
- Blue Ice