364166
MMP10 (Matrix Metalloproteinase 10, STMY2, SL2, Stromelysin 2, Transin-2)
Size
£581.00
- SKU:
- 364166
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Glu154-Ser472 of mouse MMP10, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Chang et al. (2006) found that human umbilical vein endothelial cells in which HDAC7 (HDAC7A) expression was suppressed by RNA interference failed to adhere to each other. Microarray analysis showed that HDAC7 suppression led to dysregulation of numerous genes encoding extracellular matrix and adhesion proteins, including 6.5-fold upregulation of MMP10 and 8.6-fold downregulation of the MMP10 inhibitor, TIMP1. Chang et al. (2006) found that HDAC7 downregulated MMP10 by associating with MEF2, an MMP10 transcriptional activator. Hdac7 deletion in mice, which was embryonic lethal due to widespread vascular damage, resulted in dramatic upregulation of Mmp10 in the perivascular region and downregulation of Timp1 in endothelial cells. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EGEADIM ISFAVGEHGD FYPFDGPGQS LAHAYPPGPG FYGDVHFDDD EKWTLAPSGT NLFLVAAHEL GHSLGLFHSD KKESLMYPVY RFSTSPANFH LSQDDIEGIQ SLYGAGPSSD ATVVPVLSVS PRPETPDKCD PALSFDSVST LRGEVLFFKD RYFWRRSHWN PEPEFHLISA FWPTLPSDLD AAYEAHNTDS VLIFKGSQFW AVRGNEVQAG YPKGIHTLGF PPTVKKIDAA VFEKEKKKTY FFVGDKYWRF DETRHVMDKG FPRQITDDFP GIEPQVDAVL HEFGFFYFFR GSSQFEFDPN ARTVTHILKS NS Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
- Shipping Conditions:
- Blue Ice