364127
MFNG (Beta-1,3-N-Acetylglucosaminyltransferase Manic Fringe, MFNG O-Fucosylpeptide 3-Beta-N-Acetylglucosaminyltransferase)
Size
£533.00
- SKU:
- 364127
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ile56-Tyr309 of mouse MFNG, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- MFNG is a member of the fringe gene family which also includes Radical and Lunatic fringe. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta1,3 N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The predicted 321aa mouse Mfng protein has a putative signal peptide. When misexpressed in the Drosophila wing disc, Mfng mimics some of the activities of Drosophila fringe. Expression studies of the fringe homologs in mouse embryos by in situ hybridization suggested that all 3 homologs participate in the Notch signaling pathway in determining boundaries during segmentation and cell fates during neurogenesis. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
- Shipping Conditions:
- Blue Ice