MFN1 (Mitofusin 1,Hfzo1, Hfzo2, Transmembrane GTPase MFN1, Fzo homolog)

Size

£537.00

SKU:: 364126
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Met1-Ile234 of mouse MFN1, expressed in E. coli.
Format:: Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: Mitofusin-1 is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. The deduced 741aa protein contains an N-terminal GTPase domain and a transmembrane domain near the C terminus. MFN1 shares 60% identity with MFN2, with the most extensive homology in the GTPase domains. The N- and C-terminal ends are more variable. When stably expressed in COS-7 cells, MFN1 colocalized with mitochondrial markers. Overexpression of MFN1 caused perinuclear mitochondrial clustering. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.