364111
MEPE (Matrix Extracellular Phosphoglycoprotein, OF45, Asarm Motif-Containing, Osteoblast/Osteocyte Factor 45)
Size
£524.00
- SKU:
- 364111
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ser212-Asn445 of human MEPE, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- The predicted 525aa MEPE protein has a signal peptide, 2 N-glycosylation motifs, a glycosaminoglycan-attachment site, an RGD cell-attachment motif, and phosphorylation motifs. Rowe et al. (2000) noted that MEPE shares molecular similarities with several dentin-bone extracellular matrix RGD-containing phosphoglycoproteins, including dentin sialophosphoprotein (DSPP ), osteopontin (SPP1 ), and dentin matrix protein-1 (DMP1 ). RT-PCR detected MEPE expression in bone marrow and brain. High-level expression of a 2-kb MEPE transcript was detected in 4 OHO tumor samples. Rowe et al. (2000) concluded that MEPE is a candidate gene for an OHO tumor-secreted phosphaturic factor that may play a role in the pathophysiology of tumor-induced osteomalacia. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- STHRIQHNI DYLKHLSKVK KIPSDFEGSG YTDLQERGDN DISPFSGDGQ PFKDIPGKGE ATGPDLEGKD IQTGFAGPSE AESTHLDTKK PGYNEIPERE ENGGNTIGTR DETAKEADAV DVSLVEGSND IMGSTNFKEL PGREGNRVDA GSQNAHQGKV EFHYPPAPSK EKRKEGSSDA AESTNYNEIP KNGKGSTRKG VDHSNRNQAT LNEKQRFPSK GKSQGLPIPS RGLDN Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice