364102
ME2 (Malic Enzyme 2, NADP+ Dependent, Mitochondrial)
Size
£626.00
- SKU:
- 364102
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Gly220-Ala426 of human ME2, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- ME2 encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Alternatively spliced transcript variants encoding different isoforms found for this gene. The deduced 584aa precursor protein has a calculated molecular mass of 65.4kD. The 5-prime untranslated region of the transcript contains an 80-nucleotide sequence that is extremely G/C rich (76.3%). ME2 contains a mitochondrial leader sequence, an amphiphilic alpha helix, an ADP-binding fold, and a putative dinucleotide-binding domain. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice