364095
MDA (Malondialdehyde)
Size
£563.00
- SKU:
- 364095
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Small Molecule, MDA conjugated to OVA.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- Malondialdehyde, MDA, is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism. MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA. MDA-modified proteins may show altered physico-chemical behavior and antigenicity. Malondialdehyde is a highly reactive compound that is not typically observed in pure form. In the laboratory it can be generated in situ by hydrolysis of 1,1,3,3- tetramethoxypropane, which is commercially available. It is easily deprotonated to give the sodium salt of the enolate (m.p. 245°C). Malondialdehyde reacts with deoxyadenosine and deoxyguanosine in DNA, forming DNA adducts, primarily M1G, which is mutagenic. The guanidine group of arginine residues condense with MDA to give 2-aminopyrimidines. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 100ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice