LXN (Latexin, ECI, TCI, MUM, Endogenous carboxypeptidase inhibitor, Tissue carboxypeptidase inhibitor)

Size

£531.00

SKU:: 364026
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa1-223 of rat LXN, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: Latexin is a specific inhibitor of zinc-dependent metallocarboxypeptidases. The deduced 222aa protein is an elongated molecule with N- and C-terminal domains that each consist of an alpha helix enveloped by a curved beta sheet. The 2 domains are separated by a connecting segment.Lxn was less potent against other mammalian carboxypeptidases, and it did not inhibit other metallopeptidases or serine proteases. It also showed low specificity, inhibiting several metallocarboxypeptidases containing a characteristic alpha/beta hydrolase fold. The inhibition constant was within the nanomolar range for these substrates. LXN did not inhibit enzymes of other carboxypeptidase classes. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml. Immunohistochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice