363978
LIPG (Lipase, Endothelial, EL, EDL, Endothelial Lipase, Endothelial cell-derived lipase)
Size
£581.00
- SKU:
- 363978
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- 0°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Asp115-Tyr369 of mouse LIPG expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Endothelial lipase (EL) was initially cloned by both of these laboratories using entirely different strategies. Endothelial lipase was identified as a transcript that was upregulated in cultured rat umbilical vein endothelial cells undergoing tube formation, whereas the Rader group cloned endothelial lipase as a transcript that was upregulated in the rat macrophage-like cell line THP-1 exposed to oxidized LDL. Database searches revealed that endothelial lipase shows strong sequence similarity to lipoprotein lipase (44 percent identity) and hepatic lipase (41 percent identity), two well-characterized lipases that function at vascular endothelial surfaces. Critical motifs associated with lipase activity (GXSXG and the catalytic triad S169, D193, H274), and with heparin binding were strongly conserved. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DANVVV VDWLPLAHQL YTDAVNNTRV VGQRVAGMLD WLQEKEEFSL GNVHLIGYSL GAHVAGYAGN FVKGTVGRIT GLDPAGPMFE GVDINRRLSP DDADFVDVLH TYTLSFGLSI GIRMPVGHID IYPNGGDFQP GCGFNDVIGS FAYGTISEMV KCEHERAVHL FVDSLVNQDK PSFAFQCTDS SRFKRGICLS CRKNRCNNIG YNAKKMRKKR NSKMYLKTRA GMPFKVYHYQ LKVHMFSYNN SGDTQPTLY Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice