LDHC (Lactate Dehydrogenase C, LDH-C, CT32, LDHX, LDH3, Cancer/Testis Antigen 32, LDH testis subunit)

Size

£577.00

SKU:: 363934
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Met1-Leu332 of rat LDHC expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Lactate dehydrogenase C catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHC is testis-specific and belongs to the lactate dehydrogenase family. Two transcript variants have been detected which differ in the 5' untranslated region. Lactate dehydrogenases exist in four distinct enzyme classes. Two of them are cytochrome c-dependent enzymes with each acting on either D-lactate (EC 1.1.2.4) or L-lactate (EC 1.1.2.3). The other two are NAD(P)-dependent enzymes with each acting on either D-lactate (EC 1.1.1.28) or L-lactate (EC 1.1.1.27). This article is about the NAD(P)-dependent L-lactate dehydrogenase. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSTVKEQLIQ NLAPDEKQSR CKITVVGVGN VGMACAISIL LKGLADELAL VDADENKLKG EALDLLHGSL FLSTPKIVFG KDYSVSANSK LVIITAGARM VSGESRLALL QRNVTSMKAI VPGVIQNSPD CKIMIVTNPV DILTYVVWKI SGLPVSSVIG SGCNLDSARF RYLIGEKLGV NPSSCHGWVL GEHGDSSVPI WSGVNIAGVT LKSLNPAIGS DSDKEQWKTV HKQVVDGGYE VLNLKGYTSW AIALSVTDIA ASILKNLKRV HAVTTLVKGL YGIKEEIFLS IPCVLGQSGI TDLVKVNMNT EEEALFKKSC DILWNIQKDL QL Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice