LAG3 (Lymphocyte Activation Gene 3, CD223, Protein FDC)

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£537.00

SKU:: 363892
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Asn209-Leu451 of rat LAG3 expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Lymphocyte-activation protein 3 belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. Triebel et al. (1990) identified a novel human gene of the Ig superfamily, which they designated LAG3. Expression of this gene was undetectable in resting peripheral blood lymphocytes, whereas it was found in activated T and NK cells. The corresponding cDNA encodes a 498aa membrane protein with 4 extracellular Ig-like domains. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. The 2 genes are probably derived from a common evolutionary ancestor. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice