363835
KIF5B (Kinesin Family, Member 5B, KNS1, UKHC, KINH, Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, Kinesin-1 heavy chain)
Size
£577.00
- SKU:
- 363835
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ile9-Lys226 of rat KIF5B expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Kinesins are microtubule-based motor proteins involved in the transport of organelles in eukaryotic cells. They The predicted 963aa protein has 63% sequence identity to the Drosophila KHC. Immunoblot analysis using antibodies against squid KHC detected a 120kD protein in CV-1 monkey kidney epithelial cells. Immunoblot analysis using antibodies against squid KHC detected a 120kD protein in CV-1 monkey kidney epithelial cells. Immunofluorescence studies showed that KNS1 expressed in CV-1 cells had both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules but not vimentin (VIM) intermediate filaments; the KNS1 N- and C-terminal domains, but not the alpha-helical rod domain, also colocalized with microtubules. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice