363829
KHDRBS1 (KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 Sam68, p62, GAP-Associated Tyrosine Phosphoprotein p62, Src-associated in mitosis 68kD protein, p21 Ras GTPase-activating protein-associated p62)
Size
£522.00
- SKU:
- 363829
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Lys156-Glu381 of mouse KHDRBS1 expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- SAM68 is a tyrosine-phosphorylated, SRC-associated protein in mitotic cells. SAM68 protein contains 3 N-terminal proline-rich sequences, followed by a KH domain embedded within a STAR domain, 3 more proline-rich sequences, a C-terminal tyrosine-rich region, which contains a domain similar to the SRGY domain of RNA-binding proteins, and a putative nuclear localization signal. SAM68 has a calculated molecular mass of 48.2kD, but it migrated as a 68kD protein on SDS-PAGE gels. Northern blot analysis detected a 3.1kb SAM68 transcript expressed at moderate levels in heart, brain, placenta, skeletal muscle, kidney, and pancreas, and at lower levels in lung and liver. The p62/SAM68 gene was phosphorylated when expressed in the presence of v-src, and only the phosphorylated form bound to GAP. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice