KCC4 (Potassium Chloride Cotransporters 4, SLC12A7, Solute Carrier Family 12(Potassium/ Chloride Transporters)Member 7, Electroneutral potassium-chloride cotransporter 4, K-Cl cotransporter 4)

Size

£557.00

SKU:: 363819
Application:: IHC-Fr
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa845-1056 of human KCC4 expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: By searching EST databases, Mount et al. (1999) identified a cDNA encoding SLC12A7, which they initially termed KCC3 but later renamed KCC4. The deduced 1083aa SLC12A7 protein contains 12 membrane-spanning segments, 8 phosphorylation sites, 7 of which are in the C-terminal, and 4 potential N-glycosylation sites. SLC12A7 shares 65% aa identity with SLC12A4 and 66% identity with SLC12A6. Northern blot analysis detected a 5.3kb SLC12A7 transcript in most tissues tested, with highest expression in heart and kidney and little or no expression in adult brain. Functional analysis confirmed that SLC12A7 is a KCC. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.