363818
KCC3 (Potassium Chloride Cotransporters 3, SLC12A6, ACCPN, Solute Carrier Family 12 Member 6, Potassium/Chloride Transporters, Agenesis Of Corpus Callosum And Peripheral Neuropathy, K-Cl cotranspor)
Size
£524.00
- SKU:
- 363818
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Glu559-Ser735 of human KCC3 expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Solute carrier family 12 member 6 is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The predicted 1099aa SLC12A6 protein contains 12 membrane-spanning segments and 5 N-glycosylation sites. SLC12A6 shares 77% aa identity with the ubiquitously expressed KCC1 (SLC12A4) and 73% identity with the brain-restricted KCC2. Northern blot analysis detected strong expression of 9-, 7.5-, and 4.5kb SLC12A6 transcripts in brain, heart, skeletal muscle, and kidney. Western blot analysis showed expression of a 150kD SLC12A6 protein that was reduced to 120kD by glycosidase treatment. Functional analyses confirmed that SLC12A6 is a KCC. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice