ITIH3 (Inter Alpha-Globulin Inhibitor H3, H3P, SHAP, Pre-Alpha(Globulin)Inhibitor, H3 Polypeptide, Serum-derived hyaluronan- associated protein)

Size

£626.00

SKU:: 363802
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Pro279-Val467 of human ITIH3 expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Enghild et al.(1989) found that one ITI member, the pre-alpha-trypsin inhibitor (P-alpha-I), is an approximately 130kD protein that contains the mature ITIH3 heavy chain (HC3) and a 30kD subunit. Bourguignon et al. (1993) stated that P-alpha-I is composed of HC3 covalently linked to bikunin, a Kunitz-type protease inhibitor. They reported that the 885aa H3 precursor protein contains a 10aa propeptide and a 19aa signal peptide. Before or during linkage to bikunin, the C-terminal 238aa are cleaved to produce mature HC3. H3 shares 54% and 41% identity with the H1 and H2 precursor proteins, respectively. Diarra-Mehrpour et al. (1998) corrected the cDNA sequence reported by Bourguignon et al. (1993). Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PV VPKNVAFVID ISGSMAGRKL EQTKEALLRI LEDMQEEDYL NFILFSGDVS TWKEHLVQAT PENLQEARTF VKSMEDKGMT NINDGLLRGI SMLNKAREEH RIPERSTSIV IMLTDGDANV GESRPEKIQE NVRNAIGGKF PLYNLGFGNN LNYNFLENMA LENHGFARRI YEDSDADLQL QGFYEEV Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice