363741
INSL5 (Insulin Like Protein 5)
Size
£537.00
- SKU:
- 363741
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Arg23-Cys135 of mouse INSL5 expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Insulin gene superfamily hormones regulate cell growth, metabolism, and tissue-specific functions. Members of this family are characterized by a signal peptide, a B chain, a connecting C chain, and an A chain. By EST database searching for sequences related to relaxin (RLN1), Conklin et al. (1999) identified a cDNA encoding INSL5, also designated ZINS3. The deduced 135aa protein, which shares 59% and 40% sequence identity with mouse Insl5 and human RLN1, respectively, has a dibasic cleavage site between the B and C chains. Northern blot analysis revealed expression of an approximately 1.0-kb transcript in uterus, rectum, and colon. By radiation hybrid analysis and the use of surrounding markers, Conklin et al. (1999) mapped the INSL5 gene to chromosome 1p31.1-p22.3. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-RQTVKLCG LDYVRTVIYI CASSRWRRHL EGHFHSQQAE TRNYLQLLDR HEPSKKTLEH SLPKTDLSGQ ELVRDPQAPK EGLWELKKHS VVSRRDLQAL CCREGCSMKE LSTLC Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice