363703
IL35 (Interleukin 35)
Size
£537.00
- SKU:
- 363703
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa23-215 of mouse IL35, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- Interleukin 35 (IL-35) is an IL-12 family cytokine that is produced by regulatory, but not effector, T cells and plays a role in immune suppression. It is a dimeric protein composed of IL-12alpha and IL-27beta chains, which are encoded by two separate genes called IL12A and EBI3, respectively. Secreted by regulatory T cells (Tregs), IL-35 suppresses inflammatory responses of immune cells. Studies in mice show the absence of either IL-35 chain from regulatory Tregs reduces the cells ability to suppress inflammation; this has been observed during cell culture experiments and using an experimental model for inflammatory bowel disease. To produce its suppressive effects, IL-35 has selective activities on different T cell subsets; it induces proliferation of Treg cell populations but reduces activity of Th17 cell populations. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice