363670
IL1RA (Interleukin 1 Receptor Type I, IL1RN, IL1-RA, ICIL1RA, IL1ra3, IL1F3, IRAP, IL-1RA, Interleukin-1 Family Member 3, IL1 inhibitor, Anakinra)
Size
£518.00
- SKU:
- 363670
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to His26-Gln177 of equine IL1RA, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- IL-1 receptor antagonist(IL-1RA) is a member of the Interleukin 1 cytokine family and a different type of naturally occurring inhibitor of IL-1 activity, it inhibits IL-1alpha and IL-1beta binding to interleukin receptors. A cDNA encoding this polypeptide has been isolated from monocytes and found to code for a mature 152aa residue glycoprotein of 25,000 native molecular weight. This molecule, known as secreted IL-1 receptor antagonist, shows 26%aa homology to IL-1beta and 19% homology to IL-1alpha. Evidence indicates that the inhibitory action of sIL-1ra results from binding of IL-1ra to the IL-1 receptor Type I with an affinity comparable to that of IL-1alpha or IL-1beta (Kd ~ 200 pM), thus competing with IL-1alpha or IL-1beta for binding to this receptor. This binding, however, does not result in signal transduction. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-HPLGK RPCKMQAFRI WDVNQKTFYM RNNQLVAGYL QESNTKLQEK IDVVPIEPDA LFLGLHGRKL CLACVKSGDE IRFQLEAVNI TDLSKNKEEN KRFTFIRSNS GPTTSFESAA CPGWFLCTAQ EADRPVSLTN KPKESFMVTK FYLQEDQ Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice