IGFBP2 (Insulin Like Growth Factor Binding Protein 2, IBP2, IGF-BP53)

Size

£438.00

SKU:: 363589
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Asp148-Gln325 of human IGFBP2, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Insulin-like growth factor binding protein-2 (IGFBP-2) is one of six characterized IGFBPs which specifically bind and modulate the actions of IGF-I and IGF-II. The binding affinity of IGFBP-2 for IGF-II is estimated to be significantly higher than for IGF-I, suggesting a role in regulation of IGF-II action. Studies in the fetal sheep indicate that IGFBP-2 is produced in many tissues, apparently coincident with IGF-II expression. Recent studies of serum IGFBP-2 levels have shown that, unlike for IGFBP-1, there is negligible variability during the day and, unlike IGFBP-1 and IGFBP-3, virtually no change during puberty. IGFBP-2 has also been identified in other body fluids such as cerebrospinal, amniotic and coelomic fluids, urine and seminal fluid. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice