363568
IFNa11 (Interferon Alpha 11, Limitin)
Size
£522.00
- SKU:
- 363568
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Cys24-Ala190 of mouse IFNa11, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- The weak virus-induced expression of the IFNa11 gene compared with IFNa4 was due to the presence of two nucleotide substitutions in the IFNa11 gene promoter. Irf3 or Irf7 mediated transcription and the synergism between these factors were dramatically affected in the case of IFNa11. No stimulatory effect on IFNa11 promoter transcription was observed when ikk or tbk1 was expressed alone or in the presence of irf3, although a 4-5-fold increase was obtained in the latter case, confirming that the IFNa11 gene promoter was unresponsiveness to irf3. The differential virus-induced transcription of IFNa4 and IFNa11 promoters correlated with their differential response to irf3 stimulated by ikk or tbk1. The IFNa11 gene promoter did not respond to irf3 activation even when cells expressed a constitutively active n-terminal form of rigi, whereas the IFNa4 promoter was stimulated 15-fold in similar conditions. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice