HYAL1 (Hyaluronoglucosaminidase 1, LUCA1, NAT6, Hyaluronidase-1, Lung carcinoma protein 1)

Size

£533.00

SKU:: 363533
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Ser40-Met449 of rat HYAL1, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Hyaluronan, one of the principal glycosaminoglycans of the extracellular matrix, is a high molecular weight polymer of repeating units of N-acetylglucosamine D-glucuronic acid that is synthesized in the plasma membrane of cells and translocated to the pericellular space. It is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. The predicted 435aa protein is 40% identical to the sperm-specific hyaluronidase SPAM1. Cells transfected with the HYAL1 cDNA secreted hyaluronidase activity that was biochemically indistinguishable from plasma hyaluronidase. Screening of cDNA libraries by PCR indicated that HYAL1 is expressed in multiple tissues, but not in brain. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice