HSPb7 (Heat Shock Protein Beta 7, HSP-B7, cvHSP, Cardiovascular HSP, Heat Shock 27kD Protein 7, Cardiovascular heat shock protein)

Size

£626.00

SKU:: 363525
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Met1-Ile170 of human HSPb7, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: CVHSP encodes a deduced 170aa protein with features of a small heat-shock protein, including an HSP20 (HSPB8) protein family motif and a conserved C-terminal domain. The minor CVHSP splice variants encode proteins of 175 and 68aa. Northern blot analysis detected a major transcript of 2.3 kb and minor transcripts of 1.2 and 1.6 kb in heart and skeletal muscle. RNA dot blot analysis detected high expression of CVHSP in adult and fetal heart and in skeletal muscle, with weaker expression in aorta. RT-PCR showed CVHSP expression in adipose as well. Western blot analysis detected a major 25-kD CVHSP protein and a less abundant 23-kD protein. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.