363519
HSPA12B (Heat Shock 70kDa Protein 12B, C20orf60)
Size
£517.00
- SKU:
- 363519
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Met1-Gln217 of human HSPA12B, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- The deduced proteins contains 685 and 686 aa, respectively, and have an atypical HSP70 ATPase domain, which is in 2 parts separated by a spacer region. Mouse and human HSPA12B share 93% aa identity, and human HSPA12B shares 60% identity with HSPA12A. Northern blot analysis of human tissues detected highest expression of a 3.5-kb transcript in muscle and heart, with lower expression in liver and kidney. In mouse, expression of Hspa12b was high in heart, but low or absent in all other tissues examined.both Hspa12a and Hspa12b were preferentially expressed in atherosclerotic lesions of mouse aorta. In situ hybridization confirmed that Hspa12a and Hspa12b were expressed in lesions, but not in normal nonlesional aortic tissue. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice