HSD17b14 (17-Beta-Hydroxysteroid Dehydrogenase Type 14, DHRS10, RetSDR3, SDR47C1, Retinal Short-Chain Dehydrogenase/Reductase 3, Short Chain Dehydrogenase/Reductase Family 47C,Member 1)

Size

£626.00

SKU:: 363512
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Met1-Ser270 of human HSD17b14, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: 17-beta-hydroxysteroid dehydrogenases, such as HSD17B14, are primarily involved in metabolism of steroids at the C17 position and also of other substrates, such as fatty acids, prostaglandins, and xenobiotics. The deduced 270aa protein has a calculated molecular mass of 28kD. It has all the characteristics of SDRs, including a cofactor-binding Rossman-fold domain and highly conserved motifs in the nucleotide-binding site and active site. Northern blot analysis detected HSD17B14 expression in liver, kidney, and retina.recombinant human DHRS10 converted NAD+ to NADH in the presence of estradiol, testosterone, or 5-androstene-3-beta,17-beta diol. Nonsaturable kinetics were found for testosterone, and no conversion was observed in the presence of NADPH. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice