363460
HIST2H2BE (Histone Cluster 2, H2be, H2BFQ, H2B/q, H2B.1, H2B Histone Family Member Q)
Size
£544.00
- SKU:
- 363460
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Pro2-Lys126 of human HIST2H2BE, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- HIST2H2BE encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. This intronless gene encodes a polypeptide of 126aa. This H2B gene is paired with a functional H2A histone gene, and the 2 genes are separated by a bidirectionally transcribed intergenic promoter region of 329 nucleotides containing consensus TATA and CCAAT boxes and an OTF-1 element. The 3-prime untranslated region of the H2B.1 gene contains the conserved histone 3-prime stem-loop motif commonly found in cell cycle-dependent histone genes, as well as a polyadenylation signal typical of constitutively expressed histone genes. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice