363458
HIPK2 (Homeodomain Interacting Protein Kinase 2)
Size
£626.00
- SKU:
- 363458
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ser977-Ile1198 of human HIPK2, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors.Hipk2 mRNA expression is detectable in day-15 mouse embryos and is strong in day-17 mouse embryos. The predicted 1,198aa HIPK2 protein is 96% identical to the mouse protein and has a CD95-binding site between residues 754 and 899. Northern and dot blot analyses revealed weak but ubiquitous expression of an 11.0-kb transcript that was strongest in neuronal tissue. A 7.8-kb transcript was also detected in uterus, and a strong 1.4-kb transcript was found in pancreas. Confocal microscopy demonstrated a nuclear dot localization even with a C-terminal deletion or a kinase-defective mutant. HIPK2 expressed in cells did not directly associate with CD95 or TNFR1, but did associate with TRADD. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice