GUCY1b3 (Guanylate Cyclase 1 Beta 3, GC-S-beta-1, GC-SB3, GUC1B3, GUCB3, GUCSB3, Soluble guanylate cyclase small subunit, Guanylate cyclase soluble subunit beta-3)

Size

£626.00

SKU:: 363394
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to His408-Asp619 of human GUCY1b3, expressed in E. coli.
Format:: Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha subunit and a beta subunit, typically GUCY1B3, catalyzes conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide (NO) and nitrovasodilator drugs. NOGC had been considered a soluble enzyme, but evidence that the alpha-2 subunit of the alpha-2/beta-1 isoform interacts with the PDZ domain of PSD95 (DLG4) suggests that the alpha-2 subunit directs a membrane association of this isoform. The interaction with PSD95 locates the alpha-2/beta-1 isoform in close proximity to the NO-generating NO synthase (NOS1), thereby enabling the NO sensor to respond to locally raised NO concentrations. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice