GNa11 (G Protein Alpha 11, GA11, Guanine Nucleotide-Binding Protein Subunit Alpha-11, Guanine nucleotide-binding protein G(y) subunit alpha)

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£626.00

SKU:: 363329
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Met1-Val359 of human GNa11,expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Gna11 is upstream of Gna15, and the region separating the 2 genes is 6 kb long. Phylogenetic trees revealed an approximately 6-fold higher rate of change in Gna15 than in Gna11. Mouse Gna11 and Gna15 are tandemly duplicated in a head-to-tail array. Gna11, is ubiquitously expressed, whereas expression of the downstream gene, Gna15, is restricted to hematopoietic cells. There was no evidence for alternative splicing within the coding sequence of either gene. G-alpha-q (GNAQ) and G-alpha-11 were required for maintenance of basal blood pressure and for development of salt-induced hypertension. In contrast, lack of G-alpha-12 (GNA12) and G-alpha-13 (GNA13) and their effector, Larg (ARHGEF12), did not alter normal blood pressure regulation, but blocked development of salt-induced hypertension. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice