363325
GML (Glycosylphosphatidylinositol Anchored Molecule Like Protein, LY6DL, GPI Anchored Molecule Like Protein)
Size
£626.00
- SKU:
- 363325
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Met21-Pro158 of human GML, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Furuhata et al. (1996) identified a gene encoding a predicted 158aa protein that is induced by p53. Because of its homology to glycosylphosphatidylinositol (GPI)-anchored membrane proteins, Furuhata et al. (1996) designated the gene 'GPI-anchored molecule-like protein,' or GML. By Northern blot and RT-PCR analysis, Furuhata et al. (1996) found that GML is expressed as a less than 1-kb mRNA in many tissues, including testis. Expression of GML suppressed the growth of esophageal cancer cells in culture. Furuhata et al. (1996) suggested that GML may play a role in the apoptotic pathway or cell cycle regulation induced by p53 after DNA damage.Kimura et al. (1997) found that the GML gene consists of 4 exons spanning 12 kb, with a p53-binding site 19 kb upstream of the first exon. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice