GLRX3 (Glutaredoxin 3, TXNL2, PICOT, GRX3, GLRX4, GRX4, Thioredoxin-Like 2, PKC-interacting cousin of thioredoxin, PKC-theta-interacting protein, Thioredoxin-like protein 2)

Size

£537.00

SKU:: 363309
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Glu73-Val320 of mouse GLRX3, expressed in E. coli.
Format:: Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: It has an N-terminal thioredoxin (TXN) homology domain followed by tandem repeats of an 84aa PICOT domain. Human PICOT shares 99% aa identity with the rat and mouse orthologs. The PICOT domain is highly conserved, and tandem PICOT domains are present in mouse and rat Picot. However, only a single PICOT domain is present in nematodes, yeast, bacteria, viruses, and plants. Northern blot analysis detected a 1.5-kb PICOT transcript in Jurkat cells. RT-PCR detected abundant PICOT expression in heart, spleen, and testis, with lower expression in other tissues. Western blot analysis detected PICOT at an apparent molecular mass of 38kD. Fractionation of Jurkat cells revealed PICOT in the cytosolic compartment, with very low amounts in the membrane fraction. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice