363304
GLMP (Glycosylated Lysosomal Membrane Protein, C1orf85, NCU-G1, Lysosomal protein NCU-G1)
Size
£522.00
- SKU:
- 363304
- Purity:
- Purified by Protein A and peptide affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Pro246-Asn404 of mouse GLMP.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol
- Extra Details:
- NCU-G1 is a highly glycosylated integral membrane protein of the lysosome. It is localized on chromosome 1 and consists of 6 exons. Human NCU-G1 can function as a transcription factor and as a nuclear receptor co-activator. NCU-G1 encodes a 404aa protein with a calculated molecular mass of 39kD. NCU-G1 was found to be a highly conserved nuclear protein rich in proline with a molecular weight of approximately 44kD. Its lysosomal localization was confirmed using immunofluorescence with a C-terminally His-tagged NCU-G1 and the lysosomal marker LAMP-1 as a reference, and by subcellular fractionation of mouse liver after a tyloxapol-induced density shift of the lysosomal fraction using an anti-NCU-G1 antiserum. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice