363188
GABARAPL2 (GABA-A Receptor Associated Protein Like Protein 2,
Size
£626.00
- SKU:
- 363188
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- 0°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa1-117 of human GABARAPL2, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- GABARAPL2 contains 117aa and has a calculated molecular mass of 13.7kD. Like GABARAP, it contains a basic N-terminal tubulin binding domain and a GABA-A receptor binding domain. GABARAPL2 shares 57% identity with GABARAP and 100% amino acid sequence identity with bovine brain GATE16, rat Gabarapl2, and mouse Gabarapl2. Northern blot analysis revealed ubiquitous expression of a 1.35-kb transcript with high levels in heart, brain, testis, prostate, ovary, spleen, and skeletal muscle, and low levels in lung, thymus, and small intestine. GABARAPL2 had activity as a soluble transport factor, it interacted with N-ethylmaleimide- sensitive factor (NSF) and stimulated its ATPase activity, and interacted with the Golgi v-SNARE (GOS28) in an NSF-dependent manner. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
- Shipping Conditions:
- Blue Ice