363015
F10 (Coagulation Factor X, FX, FXA, Stuart Prower Factor, Thrombokinase)
Size
£581.00
- SKU:
- 363015
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Gly21-Asn481 of mouse F10, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- As the only known physiological activator of thrombin, factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation. Human factor X is initially synthesized in the liver as a single-chain precursor of 488aa residues with a signal peptide and a pro region (residues 1 - 40). Both the intrinsic and extrinsic pathways activate factor X to Xa, which consists of light (residues 41 - 179) and heavy (residues 235 - 488) chains linked by a disulfide bond. The light chain contains a Gla and two EGF-like domains and the heavy chain corresponds to the serine protease domain. The full-length human factor X was expressed and the pro enzyme was purified and activated. The active protease was further purified and analyzed for its activity towards either peptides or proteins containing a Xa cleavage site. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice