ECE1 (Endothelin Converting Enzyme)

Size

£495.00

SKU:: 362911
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to His214-Ala448 of rat ECE1, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Endothelin converting enzyme 1, also known as ECE1, is a enzyme which in humans is encoded by the ECE1 gene.Endothelin-converting enzyme-1 is involved in the proteolytic processing of endothelin-1 (EDN1), -2 (EDN2), and -3 (EDN3) to biologically active peptides. Schmidt et al. (1994) purified a membrane-bound protease activity from bovine endothelial cells that specifically converts the inactive form to EDN1. The enzyme was cleaved with trypsin and peptide sequencing analysis confirmed it to be a zinc-chelating metalloprotease containing the typical HEXXH (HELTH) motif. RT-PCR and cDNA screens were used to isolate the complete cDNAs of the bovine and human enzymes. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- HYRTSPF FSVYVSADSK NSNSNVIQVD QSGLGLPSRD YYLNKTENEK VLTGYLNYMV QLGKLLGGGD EDSIRPQMQQ ILDFETALAN ITIPQEKRRD EELIYHKVTA AELQTLAPAI NWLPFLNAIF YPVEINESEP IVVYDKEYLR QVSTLINSTD KCLLNNYMMW NLVRKTSSFL DQRFQDADEK FMEVMYGTKK TCLPRWKFCV SDTENNLGFA LGPMFVKATF AEDSKNIA Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice