362909
EBF1 (Early B-Cell Factor 1, COE1, EBF, O/E-1, OLF1, Transcription Factor COE1)
Size
£524.00
- SKU:
- 362909
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Asp179-Ser451 of human EBF1, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- Early B-cell factor (EBF) is a tissue-specific and differentiation stage-specific DNA-binding protein that participates in the regulation of the pre-B and B lymphocyte-specific MB1 gene. Travis et al. (1993) purified the mouse Ebf protein from pre-B cells and found that it is composed of two 62-65-kD subunits. Hagman et al. (1993) determined partial amino acid sequences of Ebf and used them to isolate mouse pre-B-cell cDNAs encoding Ebf. The predicted 591aa protein has 2 functional domains: an N-terminal cysteine-rich region essential for DNA binding, and a C-terminal dimerization region containing two 15aa repeats with similarity to the dimerization domains of basic helix-loop-helix (bHLH) proteins. The calculated molecular mass of the encoded Ebf protein is 64.4kD. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- DP VIIDRFFLKF FLKCNQNCLK NAGNPRDMRR FQVVVSTTVN VDGHVLAVSD NMFVHNNSKH GRRARRLDPS EGTPSYLEHA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVIFGTMLV WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGTPG RFIYTALNEP TIDYGFQRLQ KVIPRHPGDP ERLPKEVILK RAADLVEALY GMPHNNQEII LKRAADIAEA LYSVPRNHNQ LPALANTSVH AGMMGVNSFS GQLAVNVSEA S Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice