362898
DTX1 (Deltex Homolog 1, hDx-1, E3 ubiquitin-protein ligase DTX1)
Size
£544.00
- SKU:
- 362898
- Application:
- IHC-Fr
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa290-535 of human DTX1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Expression of DTX1 partially inhibited transactivation of a CSL (RBPSUH)-dependent luciferase reporter by activated intracellular NOTCH1 (ICN1) in human and mouse cells. The N terminus of DTX1, which directly interacts with the NOTCH1 ankyrin repeats, inhibited transactivation by ICN1 possessing the ankyrin repeats, probably by inhibiting recruitment of coactivators to the C-terminal transactivation domain of NOTCH1. The 620aa DTX1 protein contains an N-terminal domain predicted to associate with Notch ankyrin repeats, followed by a proline-rich region with potential SH3 domain-binding sites and a C-terminal domain that includes a classic RING-H2 finger motif. DTX1 functioned as a ubiquitin E3 ligase. It showed highest ubiquitination activity in conjunction with the E2 enzyme UBCH5A (UBE2D1). Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice