362868
DNASEII (Deoxyribonuclease II, DNase-II, DNase2, DNASE2A, DNASE2B, DRN2, DNL, DNL2, Deoxyribonuclease II Alpha, Acid DNase, Lysosomal DNase II)
Size
£626.00
- SKU:
- 362868
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Cys19-Ile360 of human DNASEII, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Deoxyribonuclease II, or Acid DNase, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. As the name implies, it is more effective at acid pHs. There are several known DNases II, including:DNase II alpha (usually just called DNase II) ;DNase II beta (also called DLAD, or DNase II-Like Acid DNase)The predicted 360aa protein has 3 parts: a 16aa signal peptide, a 91aa propeptide, and a 253aa mature protein region. like other lysosomal enzymes, DNase II is processed by release of a signal peptide followed by proteolytic processing that generates a 2-chain enzyme. Purified DNase II migrates as 2 bands (32 and 12 kD) on SDS-PAGE. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice