DNASE1L2 (Deoxyribonuclease I Like Protein 2, DNAS1L2, DHP1, DNase I Homolog Protein DHP1, DNase I-like 2)

Size

£581.00

SKU:: 362867
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Asn28-Val232 of mouse DNASE1L2, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Germino et al. (1992) identified a DNASE1L2 exon while sequencing a cosmid from the PKD1 region on 16p13.3. Using the sequence of this exon for RT-PCR and searches of EST databases, Rodriguez et al. (1997) cloned DNASE1L2 cDNAs. The predicted 299aa DNASE1L2 protein contains a 21aa signal peptide. The aa sequence of DNASE1L2 is 56% identical to that of DNASE1. By RT-PCR, the authors detected DNASE1L2 expression in fetal and adult brain. Using RT-PCR, Shiokawa et al. (2004) detected DNASE1L2 expression in all tissues examined except colon. Peripheral blood leukocytes showed a second DNASE1L2 transcript, and by PCR of a leukocyte cDNA library, Shiokawa et al. (2004) cloned this splice variant, which they called DNASE1L2S. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice