362848
DIXDC1 (DIX Domain Containing Protein 1, CCD1, Dixin, Coiled-coil protein DIX1, DIX domain-containing protein 1)
Size
£544.00
- SKU:
- 362848
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ser485-Asn683 of human DIXDC1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- The 3-prime UTR of the cDNA contains an Alu element, and the deduced protein contains 493aa. RT-PCR ELISA detected high expression in adult whole brain and in several specific brain regions. Intermediate expression was detected in all other tissues and brain regions examined. The deduced 472aa DIXDC1 protein has a central myosin tail homology (MTH) region similar to those found in MYH1 and MYH2, as well as a C-terminal DIX domain. Within the MTH region, DIXDC1 contains 2 tyrosine phosphorylation sites and a leucine zipper motif. DIXDC1 was predicted to be a cytoplasmic protein.In contrast with human DIXDC1, the MTH region of mouse Dixdc1 has only 1 tyrosine phosphorylation site and no leucine zipper motif. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice