362825
DEFa3 (Defensin Alpha 3, Neutrophil Specific, HNP3, DEF3, HNP-2, Neutrophil defensin 2)
Size
£626.00
- SKU:
- 362825
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Pro21-Cys94 of human DEFa3, fused to N-terminal His-Tag expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- DEFA1 and DEFA3 differ from each other only by their N-terminal aa (ala and asp, respectively). DEFA2 is identical to both except that it lacks this N-terminal aa. DEFA1-, DEFA2-, and DEFA3-specific antibodies depleted antiviral activity in a dose-dependent manner, particularly against viruses using CXCR4 rather than CCR5 as a coreceptor. Addition of synthetic or purified natural defensins inhibited HIV-1 replication in vitro. Flow cytometric analysis determined that in addition to neutrophils, a small population of CD8-positive T lymphocytes harbor and secrete DEFA1, DEFA2, and DEFA3. Zhang et al. (2002) proposed that these defensins account for much of the anti-HIV-1 activity of CAF that is not attributable to beta chemokines. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice