362818
DDIT3 (DNA Damage Inducible Transcript 3, CEBPZ, CHOP, CHOP10, GADD153, C/EBP-homologous protein 10, CCAAT/enhancer-binding protein homologous protein, Growth arrest and DNA damage-inducible GADD153)
Size
£537.00
- SKU:
- 362818
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Met1-Ala168 of mouse DDIT3, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- The DDIT3 gene shows a high level of conservation in both the structural and regulatory regions to the previously described hamster gene. The human and hamster proteins share 91% aa identity. CHOP serves as a dominant-negative inhibitor of the transcription factors C/EBP and LAP. bacterially expressed CHOP inhibited the DNA-binding activity of C/EBP and LAP by forming heterodimers that could not bind DNA.Chop also activated Ero1-alpha (ERO1L), which encodes an ER oxidase. Consequently, the ER of stressed Chop -/- cells was hypooxidized relative to the ER of stressed wildtype cells. Pharmacologic and genetic manipulations promoting a hypooxidized ER reduced abnormal high molecular mass protein complexes and protected cells from the lethal consequences of ER stress. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice