DCT (Dopachrome Tautomerase, TYRP2, TRP-2, DT, Dopachrome Delta-Isomerase, Tyrosine-Related Protein 2, L-dopachrome Delta-isomerase)

Size

£524.00

SKU:: 362813
Application:: WB
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Val28-Gly263 of human DCT, expressed in E. coli.
Format:: Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: Dopachrome tautomerase, also known as DCT, is an enzyme that isomerizes the melanogenic intermediate 3,4-dihydroxyphenylalanine-chrome (DOPAchrome) to 5,6-dihydroxyindole-2-carboxylic acid (DHICA) in the biosynthesis of melanin. Its expression is regulated by the Microphthalmia-associated transcription factor. Human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region. Northern blot analysis showed that TYRP2 is expressed at high levels in human melanoma cells. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher.. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VCM TVDSLVNKEC CPRLGAESAN VCGSQQGRGQ CTEVRADTRP WSGPYILRNQ DDRELWPRKF FHRTCKCTGN FAGYNCGDCK FGWTGPNCER KKPPVIRQNI HSLSPQEREQ FLGALDLAKK RVHPDYVITT QHWLGLLGPN GTQPQFANCS VYDFFVWLHY YSVRDTLLGP GRPYRAIDFS HQGPAFVTWH RYHLLCLERD LQRLIGNESF ALPYWNFATG RNECDVCTDQ LFG Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice