CTSA (Cathepsin A, CTS-A, GLB2, GSL, NGBE, PPCA, PPGB, Protective Protein For Beta-Galactosidase, Galactosialidosis, Carboxypeptidase C, Carboxypeptidase L)

Size

£513.00

SKU:: 362745
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Leu171-Lys417 of bovine CTSA, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Cathepsin A is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. Association with CTSA, as part of the lysosomal multienzyme complex, is essential for stabilization of lysosomal beta-galactosidase (GLB1), as well as for activation of the lysosomal neuraminidase (NEU1). Galjart et al. isolated cDNA encoding the human 'protective protein.' The cDNA directed the synthesis of a 452aa precursor molecule that is processed in vivo to yield mature 'protective protein,' a heterodimer of 32kD and 20kD polypeptides held together by disulfide bridges. Because of homology to yeast carboxypeptidase Y, Galjart et al. suggested a protease activity for the 'protective protein.' Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice