362708
CRTC3 (CREB Regulated Transcription Coactivator 3, TORC3, Transducer of regulated cAMP response element-binding protein 3)
Size
£626.00
- SKU:
- 362708
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Met1-Phe103 from human CRTC3, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- By searching databases for sequences similar to TORC1 (MECT1), Iourgenko et al. (2003) identified TORC3. The deduced 618aa protein contains an N-terminal coiled-coil domain and a protein kinase A phosphorylation site. TORC3 shares 32% aa identity with TORC1. By cotransfection of TORC3 and reporter genes in HeLa cells, Iourgenko et al. (2003) found that TORC3 potently activated expression of genes driven by the IL8 promoter or a promoter carrying multiple CRE-like sequences. Forced expression of these TORCs in mouse primary muscle cells induced endogenous Pgc1-alpha and its target genes, resulting in increased mitochondrial oxidative capacity. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice